Table 1.
Concentration of proteins in biochemical reconstitution and in vivo
| Protein | Concentration in reconstitution (μM) | In vitro ratio relative to Dmc1 | Meiosis peak hour | Molecules x 104 Per cell | Concentration per cell (μM) | Concentration per nucleus (μM) | In vivo nuclear ratio relative to Dmc1 |
|---|---|---|---|---|---|---|---|
| Dmc1 | 3 | = 1.0 | 4 | 8.9 ± 1.4 | 1.74 | 24.9 | = 1.0 |
| Rad51 | 0.25 | 0.08 | 4 | 1.8 ± 0.9 | 0.36 | 5.1 | 0.2 |
| Mei5 | 0.5 | 0.17 | 4 | 5.5 ± 2.2 | 1.08 | 15.4 | 0.6 |
| Rdh54/Tid1 | 0.1 | 0.03 | 5 | 0.92 ± 0.4 | 0.18 | 2.6 | 0.1 |
| RPA | 0.2 | 0.07 | 4 | 39.2 ± 3.0 | 7.70 | 110.0 | 4.4 |
| Hop2 | 0.2 | 0.07 | 4 | 24.7 ± 2.0 | 4.84 | 69.1 | 2.8 |
For the heterodimers, only one protein concentration in the pair was determined in vivo. For the values of RPA, it is assumed that all proteins are completely localized to the nucleus.