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. 2018 Dec 4;47(2):824–842. doi: 10.1093/nar/gky1199

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — ITCH suppresses RNF168/RNF8-dependent 53BP1 foci formation by H1.2 K46 ubiquination. (A, C and E) Representative IF images of MDA-MB-231 cells, with or without IR exposure (1 Gy, 4 h), using the indicated antibodies against proteins involved in DNA damage and DAPI counterstaining for nuclei; (B, D and F) Quantification of foci/nucleus in 30 nuclei from each group, data are represented as mean ± S.D., P values compared between groups indicated with a bracket. (A and B) cells transfected with shITCH for ITCH knockdown or scrambled shRNA control; (C and D) cell transfected with single or combined shRNA knockdown plasmids against ITCH (shITCH), RNF8 (siRNF8), or RNF168 (siRNF168); (E and F) cells overexpressing GFP-WT or GFP-K46R-H1.2, N = 3.