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. Author manuscript; available in PMC: 2019 Jan 24.
Published in final edited form as: J Med Chem. 2018 May 7;61(10):4593–4607. doi: 10.1021/acs.jmedchem.8b00389

Figure 4.

Figure 4.

(A) Neuroprotection concentration response curve for 5a. PC12 cells were submitted to OGD for 90 min followed by addition of 5a (0.1, 1, 5, 10, 25, or 50 μM) and incubated under normoxic conditions for 24 h. Data represent percent change in viability relative to vehicle-treated control mean = 1, Prestoblue readout. Vehicle control viability range (assay baseline [blue]) and viability range control plates not submitted to OGD are shown in yellow. (B) Neuroprotection of 5a is dependent on sGC signaling. ODQ (10 μM) abolished neuroprotective activity of 5a in a PC12-OGD assay using MTT as a viability readout. Data represented as mean ± SEM (n = 3) of duplicate experiments. *p < 0.05, **p < 0.01, ***p < 0.001 compared to vehicle control using one-way ANOVA analysis with Dunnett’s posthoc test. Average viability of the OGD vehicle cells was51.4% (±4.1) that of vehicle cells based on 20 OGD/vehicle trials, represented as mean ± SEM.

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