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. 2018 Nov 12;47(2):929–940. doi: 10.1093/nar/gky1153

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Sedimentation coefficient distribution of C. crescentus ProRS, C. crescentus YbaK, E. coli tRNA^Pro and their complexes. (A) AUC results of individual species. ProRS (blue) absorbance at 280 nM, AF680-YbaK (cyan) absorbance at 679 nM, and tRNA^Pro (red) absorbance at 256 nM. (B) ProRS alone (blue), tRNA^Pro (red), and ProRS mixed with tRNA^Pro (pink). (C) AF680-YbaK (cyan), AF680-YbaK mixed with ProRS (blue), AF680-YbaK mixed with tRNA^Pro (red) and AF680-YbaK mixed with ProRS and tRNA^Pro (green).