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. 2018 Nov 12;47(2):929–940. doi: 10.1093/nar/gky1153

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Native mass spectra and ion mobiligram of the binary complex of ProRS/tRNA^Pro. (A) Mass spectrum of a mixture of ProRS and tRNA^Pro. (B) Zoom in of the high m/z region (5300–6800 m/z) of (A) showing the complex of two ProRS with one tRNA^Pro; the observed mass of the binary complex is 124.5 kDa whereas the theoretical mass is 123.8 kDa. (C) Ion mobiligram illustrating products after SID (100 V; 1900 eV) of the binary complex. * represents the precursor peak that was isolated for SID (adjacent peaks are from charge stripping and reflect different charged states). ProRS monomer peaks are indicated and ProRS/tRNA complex peaks are circled.