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. 2018 Nov 12;47(2):929–940. doi: 10.1093/nar/gky1153

Figure 8.

Figure 8.

Cys-tRNA deacylation by YbaK or the YbaK/ARS complex in the absence and presence of EF-Tu. Deacylation reactions containing 0.5 μM Cys-tRNA, 0.1 μM YbaK, 0.5 μM ARS and 0.5 μM EF-Tu or BSA were carried out at 37°C. (A) Cys-tRNAPro deacylation by YbaK alone (red, dotted line), YbaK·ProRS (red, solid line), YbaK with EF-Tu (blue, dotted line), and YbaK·ProRS with EF-Tu (blue, solid line). (B) Cys-tRNACys deacylation by YbaK alone (red, dotted line), YbaK·CysRS (red, solid line), YbaK with EF-Tu (blue, dotted line), and YbaK·CysRS with EF-Tu (blue, solid line). Controls with buffer or ARS alone are shown in black solid and dashed lines, respectively.