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. 2018 Nov 12;8(2):169–178. doi: 10.1002/sctm.15-0399

Table 1.

List of primers used for reverse transcriptase‐polymerase chain reaction

Gene Primer sequence (5′–3′) Temperature (°C)
Nestin Forward: AATGGGAGGATGGAGAATGGAC 56
Reverse: TAGACAGGCAGGGCTAAGCAAG
Musashi‐1 Forward: GGCTTCGTCACTTTCATGGACC 58
Reverse: GGGAACTGGTAGGTGTAACCAG
Sox‐2 Forward: TACCTCTTCCTCCCACTCC 56
Reverse: GATTGCCATGTTTATCTCG
Nanog Forward: AGGGTCTGCTACTGAGATGCTCTG 56
Reverse: CAACCACTGGTTTTTCTGCCACCG
Oct3/4 Forward: GCCAGGCTCCTGATCAACAGCATCA 56
Reverse: ATGGCTGGACACCTGGCTTCAGACT
OMP Forward: CCCAGCAGATGCGGCTCCGA 66
Reverse: GATCAGAAAGGCGCTCCCCA
GAPDH Forward: ACCACAGTCCATGCCATCAC 58
Reverse: TCCACCACCCTGTTGCTGTA