Table 1.
Gene | Primer sequence (5′–3′) | Temperature (°C) | |
---|---|---|---|
Nestin | Forward: | AATGGGAGGATGGAGAATGGAC | 56 |
Reverse: | TAGACAGGCAGGGCTAAGCAAG | ||
Musashi‐1 | Forward: | GGCTTCGTCACTTTCATGGACC | 58 |
Reverse: | GGGAACTGGTAGGTGTAACCAG | ||
Sox‐2 | Forward: | TACCTCTTCCTCCCACTCC | 56 |
Reverse: | GATTGCCATGTTTATCTCG | ||
Nanog | Forward: | AGGGTCTGCTACTGAGATGCTCTG | 56 |
Reverse: | CAACCACTGGTTTTTCTGCCACCG | ||
Oct3/4 | Forward: | GCCAGGCTCCTGATCAACAGCATCA | 56 |
Reverse: | ATGGCTGGACACCTGGCTTCAGACT | ||
OMP | Forward: | CCCAGCAGATGCGGCTCCGA | 66 |
Reverse: | GATCAGAAAGGCGCTCCCCA | ||
GAPDH | Forward: | ACCACAGTCCATGCCATCAC | 58 |
Reverse: | TCCACCACCCTGTTGCTGTA |