Figure 1.

Generation of human pluripotent stem cell‐derived hepatocytes and their therapeutic potential in various model systems. (A): Four‐stage hepatic differentiation from human pluripotent stem cells (hPSC) to definitive endoderm, hepatic endoderm, and subsequently hepatocytes (hPSC‐Heps) over a 21‐day protocol by a defined cocktail of growth factors and small molecules (in the box). (B): Schematic of further maturation of hPSC‐Heps in two‐dimensional culture dish coated with type I collagen, three‐dimensional (3D) inverse colloidal crystal scaffold coated with type I collagen (inverse colloidal crystal) and encapsulation within 3D alginate microspheres.