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. 2018 Nov 4;8(2):179–193. doi: 10.1002/sctm.18-0026

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© 2018 The Authors. stem cells translational medicine published by Wiley Periodicals, Inc. on behalf of AlphaMed Press

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Localization of Ca_V1.3 during hESC‐RPE maturation. Immunolabeling of Ca_V1.3 (green) together with centrosome protein PCNT (red) from post‐confluence day 1 to post‐confluence day 84 at four time points: (A) day 1, (B) day 6, (C) day 31, and (D) day 84 (cell line 08/017). (E): Labeling acetylated α‐tubulin (red) together with Ca_V1.3 (green) shows the localization of Ca_V1.3 at the base of the primary cilia during maturation (cell line 08/017, days post‐confluence 40). The confocal images are shown as xy‐maximum intensity projections and yz‐confocal sections (apical side upwards, localization of the section highlighted with a white bar). Scale bars 10 μm. Abbreviations: Ca_V, voltage‐gated Ca²⁺ channel; hESC, human embryonic stem cell; RPE, retinal pigment epithelium; PCNT, pericentrin.