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. 2018 Nov 1;8(2):112–123. doi: 10.1002/sctm.18-0036

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© 2018 The Authors stem cells translational medicine published by Wiley Periodicals, Inc. on behalf of AlphaMed Press

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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A highly efficient strategy for generating mRNA‐induced midbrain dopaminergic (miDA) neurons from Induced pluripotent stem cells (iPSCs). (A): Diagram of the differentiation protocol (S/F/D: SHH, FGF8b, and DAPT). (B): Brightfield microscopic images show iPSC1 cells at day 0, 5, and 8 of differentiation (scale bar: 100 μm). (C): Three iPSC lines were differentiated following the protocol shown in (A). Cells were counted to calculate the number of neuronal precursor cells (NPCs) and neurons at day 5 and 8 of differentiation, respectively. Data represents mean ± SEM (n = 6).