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. 2018 Nov 1;8(2):112–123. doi: 10.1002/sctm.18-0036

Figure 5.

Figure 5

6‐Hydroxydopamine (6‐OHDA) induces neurotoxicity in mRNA‐induced midbrain dopaminergic (miDA) neurons. (A): miDA neurons derived from iPSC1 cells were grown in 384‐well plates and stained with Calcein AM after 3, 5, and 15 days of in vitro maturation (scale bar: 1000 μm). (B, C): Neurite tracing (B) and quantification (C, n = 6) shows increase of neurite length in miDA neurons during in vitro maturation (scale bar: 100 μm). (D, E): miDA neurons after 5 days of maturation received 6‐OHDA or mock treatment for 24 hours. Calcein‐AM‐stained neurons were subjected to neurite length quantification (scale bar: 1,000 μm [×4] and 100 μm [×20]). Data represents mean ± SEM. *p < .01.