Figure 3.

The RT-PCR was used to analyze a subset of genes that were differentially regulated in both the in vitro EB system and in the developing ovary. Microarray parameters highlighted genes with ≥2-fold change in expression. Of 25 analyzed genes, 14 were actively expressed at all developmental ages (A). Fifteen genes demonstrated a temporal shift in expression-related stage of ovarian development (B). Localization of genes present in adult ovarian tissue by RT-PCR was specific to mural GCs and not found in other ovarian tissues (select examples, C-J). Scale bar: 100 µm. EB indicates embryoid body; GCs, granulosa cells; RT-PCR, reverse transcription polymerase chain reaction.