Table 1.

Purification of recombinant wild-type and mutant ALAS2^a

ALAS2 enzyme	Step	Activity	Specific.activity	Yield	Fold Purification
		Units	Units/mg	%	fold
Wild type	Crude extract	571,000	1130	100	1
	Affinity chromatography	323,000	21,500	56	20
	Gel filtration	131,000	93,500	23	83
p.Arg559His	Crude Extract	1,090,000	2090	100	1
	Affinity chromatography	358,000	29,600	33	14
	Gel filtration	95,600	108,000	8.8	52
p.Glu565Asp	Crude extract	1,030,000	1710	100	1
	Affinity chromatography	825,000	50,200	80	29
	Gel filtration	194,000	122,000	19	71
p.Arg572Cys	Crude extract	267,000	780	100	1
	Affinity chromatography	143,000	8760	53	11
	Gel filtration	43,500	121,000	16	155
p.Ser573Phe	Crude extract	666,000	1070	100	1
	Affinity chromatography	301,000	11,400	45	11
	Gel filtration	84,200	115,000	13	107
p.Tyr586Phe	Crude Extract	845,000	1650	100	1
	Affinity chromatography	287,000	15,800	34	10
	Gel filtration	128,000	109,000	15	65
P561X	Crude Extract	4,660,000	8990	100	1
	Affinity chromatography	3,110,000	156,000	66	17
	Gel Filtration	1,730,000	288,000	37	32
V562X	Crude Extract	6,410,000	13,300	100	1
	Affinity chromatography	2,960,000	116,000	46	9
	Gel filtration	1,330,000	402,000	21	30
H563X	Crude Extract	3,480,000	9070	100	1
	Affinity chromatography	2,990,000	170,000	86	19
	Gel Filtration	1,610,000	300,000	46	33
F575X	Crude Extract	1,510,000	2990	100	1
	Affinity chromatography	355,000	30,500	23	10
	Gel Filtration	174,000	134,000	12	45

^aData are averages from at least 3 independent purifications