Fig. 3.

DSBs were increased by CKI treatment. a γ-H2AX expression from 3 to 48 h after treatment with 2 mg/ml CKI in two cell lines. b Localization of γ-H2AX in two cell lines after CKI treatment for 48 h. Blue is DAPI staining of nuclei, pink/red is staining of DSBs with antibody to γ-H2AX. The bar graph shows a quantification of the average number of γ-H2AX foci per cell detected in immunofluorescence images of 2 mg/ml CKI treated and untreated groups of 3 independent replicate experiments. c Expression of DSBs repair proteins, Ku70 and Ku80, as a result of treatment with 2 mg/ml CKI in two cell lines. Statistical analyses were performed using two-way ANOVA or t-test (for microscopy) comparing treated with untreated (*p <0.05, **p <0.01, ***p <0.001, ****p <0.0001); bars show one standard deviation from the mean