Table 3.

Qualitative and quantitative validation of iYL1539

Substrates	In vivo	In silico	References
Prediction of growth phenotypes of Alternaria sp. MG1 on different carbon and nitrogen sources
Carbon sources
Glucose	+	+	[24]
Fructose	+	+	[24]
Galactose	+	+	[86]
Lactose	+	+	[24]
Maltose	+	+	[86]
Mannose	+	+	[86]
Pectin	+	−	[86]
Ribose	+	+	[86]
Starch*	+	+	[24]
Sucrose*	+	+	[24]
Xylose	+	+	[86]
Arabinose	+	+	[38]
Rhamnose*	+	+	[38]
Raffinose	+	+	[38]
Glycerol	+	+	[87]
Dextrin*	+	+	[24]
Citrate	+	−	[87]
Polygalacturonic acid	+	−	[88]
Cellobiose	+	+	[89]
Sorbose	+	+	[89]
Nitrogen sources
NH4Cl	+	+	[24]
KNO3	+	+	[24]
NaNO2	+	+	[86]
Acetamide	+	−	[38]
Urea	+	+	[24]
Glycine	+	+	[89]
Glutamate	+	+	[89]
Arginine	+	+	[89]
Alanine	+	+	[89]
Aspartate	+	+	[89]
Asparagine	+	+	[89]
Tyrosine	+	+	[89]

Comparison of in silico and in vivo growth rates
Constraints (mmol/gDW/h)		Growth rate (h−1)
Consumption rate	RES synthesis rate	In vivo	In silico
Glc (v = 1.65)	8.36E−6	0.1000	0.1013
Suc (v = 0.73)	1.50E−6	0.0893	0.0885

Glc glucose, Suc sucrose, RES resveratrol

+ for growth/− for non-growth; * represents Alternaria sp. MG1 can utilize after filling gaps