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. 2019 Jan 23;20:7. doi: 10.1186/s12865-019-0287-y

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — IL-6 expression levels of organs in Ceacam1^−/− mice in response to LPS challenge. (a) IL-6 mRNA expression levels of mouse organs after i.p. injection of LPS (10 mg/kg) for 2 h (n = 4). (B) IL-6 mRNA expression level in PBS perfused liver and splenocytes after treated with 500 ng/mL LPS for 2 h in vitro (n = 3). (c) IL-6 mRNA expression level in bone marrow cells after treated with 500 ng/mL LPS for 2 h in vitro (n = 3). (d) Percentage of bone marrow CD11b⁺Ly6G⁻ cells in WT and Ceacam1^−/− mice (n = 4). (e) Intracellular staining of IL-6 in bone marrow cells after treated with Brefeldin A (BFA) plus 500 ng/mL LPS for 5 h in vitro (n = 3). (f) Intracellular staining of IL-6 of bone marrow cells after i.p. injection of BFA plus LPS (10 mg/kg) for 5 h in vivo (n = 3). (g) Intracellular staining of IL-6 of splenocytes after i.p. injection of BFA plus LPS (10 mg/kg) for 5 h in vivo (n = 3)