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. 2019 Jan 24;20:20. doi: 10.1186/s13059-019-1628-0

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Screenings for essential genes involved in TcdB toxicity at MOI of 0.3, 3, and 10 in HeLa cells. a, b Screening scores of identified genes (FDR < 0.15) calculated by MAGeCK (a) and by MAGeCK^iBAR (b) at MOI of 0.3. c, d Screening scores of identified genes (FDR < 0.15) calculated by MAGeCK (c) and by MAGeCK^iBAR (d) at MOI of 3. e, f Screening scores of identified genes (FDR < 0.15) calculated by MAGeCK (e) and by MAGeCK^iBAR (f) at MOI of 10. Negative control genes are labeled with black dots. Rankings of identified candidates in each biological replicate through MAGeCK and MAGeCK^iBAR were presented