Fig. 5.

Treatment with siB2M or siCtrl does not influence expression of pluripotency factors in hES cells. a Flow cytometric analysis of core pluripotency factors expression at protein level at 72 h in cells treated with 20 nM siRNA, represented as percentage of positive cells for OCT4 and NANOG, and as a protein level (estimated according to gMFI change) relative to untreated sample for SOX2. For gating strategy, see the “Methods” section. b RT-qPCR analysis of representative pluripotency and differentiation markers’ expression upon treatment with siOCT4, siB2M, or siCtrl. mRNA level is presented as logarithm base 2 of the fold change in gene expression between untreated and siRNA treated sample. Statistical significance with P values less than 0.05 are labeled as * (mean ± SEM, N = 3). Abbreviations: UT, untreated