Table 1.
Antibodies used for flow cytometric analysis and immunofluorescence microscopy
| Name | Dilution | Host and clonality | Supplier | |
|---|---|---|---|---|
| Target protein | ||||
| OCT4 (Alexa 647) | 653710 (3A2A20) | 1:100 | Mouse monoclonal IgG2b | BioLegend |
| NANOG (PE) | 560483 (N31-355) | 1:5 | Mouse monoclonal IgG1 | BD Biosciences |
| SOX2 (PerCp-Cy5.5) | 561506 (030-678) | 1:20 | Mouse monoclonal IgG1 | BD Biosciences |
| B2M (Alexa 488) | FAB8248G (883028) | 1:20 | Mouse monoclonal IgG1 | R&D Systems |
| Isotype controls | ||||
| Alexa 647 conjugate | 400330 | 1:100 | Mouse IgG2b | BioLegend |
| PE conjugate | 554680 | 1:80 | Mouse IgG1 | BD Biosciences |
| PerCp-Cy5.5 conjugate | 552834 | 1:5 | Mouse IgG1 | BD Biosciences |
| Alexa 488 conjugate | 557702 | 1:25 | Mouse IgG1 | BD Biosciences |
Fluorescent label conjugated to antibody is indicated in the brackets of target protein column. Abbreviations: PE phycoerythrin, PerCP peridinin chlorophyll protein complex