Table 2.

Primers used in polymerase chain reaction

Gene name	Primer sequence
B2M	Forward 5′-TGCTGTCTCCATGTTTGATGTATCT-3′
	Reverse 5′-TCTCTGCTCCCCACCTCTAAGT-3′
BRA	Forward 5′-ATGACAATTGGTCCAGCCTT-3′
	Reverse 5′-CGTTGCTCACAGACCACAG-3′
GATA4	Forward 5′-GCTCCTTCAGGCAGTGAGAG-3′
	Reverse 5′-CTGTGCCCGTAGTGAGATGA-3′
HAND1	Forward 5′-TGCCTGAGAAAGAGAACCAG-3′
	Reverse 5′-ATGGCAGGATGAACAAACAC-3′
NANOG	Forward 5′-CCTGTGATTTGTGGGCCTG-3′
	Reverse 5′-GACAGTCTCCGTGTGAGGCAT-3′
OTX2	Forward 5′-GCTGGCTATTTGGAATTTAAAGG-3′
	Reverse 5′-GGGTTTGGAGCAGTGGAAC-3′
PAX6	Forward 5′-AACAGACACAGCCCTCACAAAC-3′
	Reverse 5′-CGGGAACTTGAACTGGAACTGAC-3′
POU5F1 (OCT4)	Forward 5′-CTGGAGCAAAACCCGGAGG-3′
	Reverse 5′-CCTCAAAGCGGCAGATGGTC-3′
RPL13A	Forward 5′-CCTGGAGGAGAAGAGGAAAGAGA-3′
	Reverse 5′-TTGAGGACCTCTGTGTATTTGTCAA-3′
SOX1	Forward 5′-CAGCAGTGTCGCTCCAATTCA-3′
	Reverse 5′-GCCAAGCACCGAATTCACAG-3′
SOX2	Forward 5′-GTATCAGGAGTTGTCAAGGCAGAG-3′
	Reverse 5′-TCCTAGTCTTAAAGAGGCAGCAAAC-3′
SOX17	Forward 5′-GGCGCAGCAGAATCCAGA-3′
	Reverse 5′-CCACGACTTGCCCAGCAT-3′
TBP	Forward 5′-TGCACAGGAGCCAAGAGTGAA-3′
	Reverse 5′-CACATCACAGCTCCCCACCA-3′