Figure 4.

DC loaded TNBC TCL can induce CTL emergence in PBL. Purified mouse DCs were cultured for 6 days with granulocytemacrophage colonystimulating factor and IL4 in a medium containing P65-1187TCL, P65-231TCL, P65-1937TCL, P65-436TCL, or 1×PBS. On day 7, DCs were cocultured with PBL from different donors. After 48 h, the PBL were stained with FITC-labeled anti-CD8 and PE-labeled anti-IFN-γ mAb to conduct flowcytometry. Numbers in each plot represent the percentage of CD8 and IFN-γ double-positive cells in PBL.