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Apoptosis detection of PBMC induced by TNBC TCL through PD/PDL-1 interaction. PBMC were cultured for 48 h in medium containing 1187TCL, 231TCL, 1937TCL, and 436TCL, 1×PBS or each TNBC TCL plus anti-PD antibody. After 48 h, the apoptosis of PBMCs was determined by flow cytometry. Numbers in each plot represent the percentage of propidium iodide (PI) and annexin V double-positive cells. Representative data from 1 of 3 experiments is shown.
