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. Author manuscript; available in PMC: 2019 Jan 24.
Published in final edited form as: Immunity. 2018 Nov 13;49(6):1116–1131.e7. doi: 10.1016/j.immuni.2018.10.013

Figure 5. LGG and Butyrate (But) Do Not Induce Bone Anabolic Effects in Mice that Are Deficient in T Cell Production of Wnt10b.

Figure 5.

TCRβ−/− mice were adoptively transferred with WT CD4+ T cells and WT CD8+ T cells, or WT CD4+ T cells and Wnt10b−/− CD8+ T cells.

(A) Whole BM Wnt10b transcript levels.

(B) Wnt10b transcript levels in CD8+ T cells purified from reconstituted TCRβ−/− mice at the end of the LGG or butyrate (but) treatment period.

(C) Prospective measurements of vertebral trabecular bone volume fraction (BV/TV) by in vivo μCT scanning.

(D) Femoral BV/TV as measured by in vitro μCT scanning at the end of the treatment period.

(E) Serum levels of osteocalcin, a marker of bone formation.

n = 5–12 mice per group. In (B), samples from 2 mice were pooled together. Data are expressed as mean ± SEM. All data were normally distributed according to the Shapiro-Wilk normality test. Data were analyzed by two-way ANOVA for repeated-measures and post hoc tests applying the Bonferroni correction for multiple comparisons. *p < 0.05, **p < 0.01, and ****p < 0.0001 compared to the indicated group, or baseline. ##p < 0.01, ###p < 0.001 compared to vehicle. ns = not significant.