| Antibodies |
|
|
|
InVivomAb anti-CD25 Ab (clone
PC61) |
BioXCell |
Cat# BE0012; RRID: AB_1107619 |
|
InVivomAb anti-CD25 isotype
matched irrelevant Ab |
BioXCell |
Cat# BE0088; RRID: AB_1107775 |
| Anti-Mouse BV 711-CD8-(clone
53–6.7) |
Biolegend |
Cat# 100747; RRID: AB_11219594 |
| Anti-Mouse CD16/32 (Clone 93) |
Biolegend |
Cat# 101302; RRID: AB_312801 |
| Anti-Mouse BV 421-TCRβ (clone
H57–597), |
Biolegend |
Cat# 109230; RRID: AB_2562562 |
| Anti-Mouse PerCP/Cy5.5-CD4 (clone
RM4–5) |
Biolegend |
Cat# 100540; RRID: AB_893326 |
| Anti-Mouse PE-CD25 (clone PC61) |
Biolegend |
Cat# 102008; RRID: AB_312857 |
| Anti-Mouse PerCP/Cy5.5-F4/80 (clone BM8) |
Biolegend |
Cat# 123128; RRID: AB_893484 |
| Anti-Mouse BV 421-CD11c (clone N418) |
Biolegend |
Cat# 117330; RRID: AB_11219593 |
| Anti-Mouse Alexa Fluor 700-I-A/I-E (clone
M5/114.15.2) |
Biolegend |
Cat# 107622; RRID: AB_493727 |
| Anti-Mouse PE-CD40 (clone 3/23) |
Biolegend |
Cat# 124610; RRID: AB_1134075 |
| Anti-Mouse APC-CD80 (clone
16–10A1) |
Biolegend |
Cat# 104714; RRID: AB_313135 |
| Anti-Mouse BV650-CD86 (clone GL1) |
BD Biosciences |
Cat# 564200; RRID: AB_2738665 |
| Anti-Mouse APC-anti-Foxp3 (clone FJK-16
s) |
eBioscience |
Cat# 17–5773; RRID: AB_469457 |
| Anti-Mouse PE anti-AKT (pS473) |
BD Biosciences |
Cat# 560378; RRID: AB_1645328 |
| anti-NFAT1 |
ThermoFisher Scientific |
Cat# MA1–025; RRID: AB_2152763 |
| anti-c-Jun |
ThermoFisher Scientific |
Cat# 397500; RRID: AB_2533433 |
| anti-NFAT2 |
Santa Cruz Biotechnology |
Cat# sc-7294; RRID: AB_2152503 |
| anti-c-Fos |
Santa Cruz Biotechnology |
Cat# sc-52-G; RRID: AB_2629503 |
| anti-phospho-Smad2 (Ser465/467) |
Cell Signaling Technology |
Cat# 3108; RRID: AB_490941 |
| anti-phospho-Smad3 (Ser423/425) |
Cell Signaling Technology |
Cat# 9520; RRID: AB_2193207 |
| anti-Smad3 (phospho S423+ S425) |
Abcam |
Cat# ab52903; RRID: AB_882596 |
| Anti-beta Tubulin |
Abcam |
Cat#. 6046; RRID: AB_2210370 |
| anti-Lamin B1 |
Cell Signaling |
Cat# 12586; RRID: AB_2650517 |
| Anti-beta actin |
Santa Cruz Biotechnology |
Cat# sc-1616; RRID: AB_630836 |
| phospho-PI3K p85 |
Cell Signaling Technology |
Cat# 4257; RRID: AB_659889 |
| Control rabbit IgG |
ThermoFisher Scientific |
Prod# 1862244 |
| Anti CD3 Ab |
biolegend |
Cat# 100331; RRID: AB_1877073 |
| Mouse CD4+ cell isolation kit |
STEMCELL Technologies |
Cat# 19852A |
| Mouse CD8a Selection kit II |
STEMCELL Technologies |
Cat# 18953 |
| Mouse T cell isolation kit |
STEMCELL Technologies |
Cat# 19851A |
| Mouse CD11c isolation kit |
Miltenyi Biotech |
Cat# 130–108–338 |
| CTLA4Ig (Abatacept) |
Bristol-Myers Squibb Company |
NA |
| Bacterial and Virus Strains |
|
|
|
Lactobacillus rhamnosus
GG |
American Type Tissue Collection |
ATCC 53103 |
| Chemicals, Peptides, and Recombinant
Proteins |
|
|
| Diphtheria Toxin |
Merck |
Cat# 322326 |
| Butyrate |
Cayman Chemicals |
Cat# 156–54–7 |
| acetonitrile-water (1:1, v/v), |
Fisher Chemical |
Cat# A4522 |
| Butyrate standard |
Sigma |
Cat# 08089 |
| 3-nitrophenylhydrazine hydrochloride |
Sigma |
Cat# N21804
|
| N-(3-dimethylaminopropyl)-N”-ethylcarbodiimide
hydrochloride |
Sigma |
Cat# 03449 |
| 6% pyridine |
Sigma |
Cat # 270407 |
| 0.1% formic acid |
ThermoFisher scientific |
Cat # 85178 |
| NE-PER Nuclear and Cytoplasmic Extraction
Reagents |
ThermoFisher Scientific |
Cat# 78833 |
| Halt Protease and Phosphatase Inhibitor
Cocktail |
ThermoFisher Scientific |
Cat# 78441 |
| rpmi-1640 medium |
Mediatech, Inc. |
Cat# 10–040-CV |
| Recombinant human IL-2 |
Biolegend |
Cat# 575408 |
| TGFβ1 |
Peprotech |
Cat# 100–21 |
| Trans-retinoic acid |
ThermoFisherScientific |
Cat# AC20734–0010 |
| Ionomycin |
Sigma |
Cat# 13909 |
| OVA peptide antigen |
Andspec |
Cat# AS-60193 |
| Q5 Site-Directed Mutagenesis |
BioLabs |
Cat# E0554S |
| Critical Commercial Assays |
|
|
| EasySep Mouse CD4+ and CD8+ T Cell Isolation
Kit |
StemCell Technologies |
Cat# 19852 |
| P1NP ELISA |
Immunodiagnostic Systems |
Cat# AC-33F1 |
| Osteocalcin ELISA |
Immunodiagnostic Systems |
Cat# AC-12F1 |
| CTX ELISA |
Immunodiagnostic Systems |
Cat# AC-06F1 |
| BrdU ELISA kit |
Roche Diagnostics |
Cat# 11 647 229 001 |
| CaspACE Assay System |
Promega |
Cat# G7351 |
| LIVE/DEAD Fixable Yellow Dead Cell Stain
Kit |
ThermoFisher |
Cat# L34959
|
| NE-PER Nuclear and Cytoplasmic Extraction
Reagents |
ThermoFisher Scientific |
Cat# 78833 |
| Luminata Crescendo Western HRP substrate |
EMD Millipore |
Cat# WBLUR0100 |
| Amaxa Mouse T Cell Nucleofector Kit |
Lonza |
Cat# V4XP-3012 |
| Dual-Luciferase reporter assay kit |
Promega |
Cat# E1910 |
| EasySep Mouse CD8a positive Selection Kit
II |
StemCell Technologies |
Cat# 18953 |
| Pierce Agarose ChIP Kit |
ThermoFisher Scientific |
Cat# 26156 |
| ABI SYBR Green PCR master mix |
Applied Biosystems |
Cat# 4309155 |
| Experimental Models: Organisms/Strains |
|
|
| Mouse: C57BL/6 |
Jackson Laboratory |
Cat# 000664;RRID:IMSR_JAX:000664 |
| Mouse: (TCRβ−/−)
B6.129P2-Tcrbtm1Mom/J |
Jackson Laboratory |
Cat# 002118;RRID:IMSR JAX:002118 |
| Mouse: (OT-1)
C57BL/6-Tg(TcraTcrb)1100Mjb/J |
Jackson Laboratory |
Cat# 003831;RRID:IMSR JAX:003831 |
| Mouse: (DEREG) C57BL/6-Tg(Foxp3-DTR/EGFP)
23.2Spar/Mmjax |
Jackson Laboratory |
Cat# 32050-JAX;RRID:MMRRC 032050-JAX |
| Oligonucleotides |
|
|
| Primers for qRT-PCR and ChIP-qPCR, see Table
S1 |
Invitrogen |
N/A |
| Recombinant DNA |
|
|
| pGL3.0 vector |
Promega |
N/A |
| pGL3.0 –2000 to +216 bp DNA sequence of
mouse Wnt10b |
Roberto Pacifici, Emory University SOM (This
Paper) |
N/A |
| pGL3.0 |
Promega |
N/A |
| mWnt10b-luc (a plasmid containing 705 to +216
bp DNA sequence of mouse Wnt10b promoter constructed by inserting a PCR
product of mouse genomic DNA into the pGL3.0 vector) |
Gift from Dr. D. J. Klemm,University of
Colorado,Denver. (PMID:18957421) |
N/A |
| Software and Algorithms |
|
|
| MultiQuant 3.0.2 software |
AB Sciex, USA |
https://sciex.com/products/software/multiquant-software |
| Graphpad Prism 6 |
GraphPad Software |
N/A |
| Bioquant Image Analysis System |
(R&M Biometrics) |
https://osteo.bioquant.com/ |
| FlowJo |
Tree Star, Inc. |
https://www.flowjo.com/ |
| Quantity One 1D Analysis Software |
Bio-Rad Laboratories |
http://www.bio-rad.com/en-us/product/quantity-one-1-d-analysis-software?ID=1de9eb3a-1eb5-4edb-82d2-68b91bf360fb |
| Other |
|
|
| 1.4 mm ceramic beads |
Fisher Scientific |
Cat# 15340153 |
| C18-reverse phase HPLC Accucore column (4.6
× 100mm, 2.6mm) |
ThermoScientific, USA |
Cat# 17126–032130 |
| MACS Microbeads |
Miltenyi Biotec |
Cat# 130–106–639 |
