Fig. 3.

Compound 14a has no significant effect on related TRP channels. Stable HEK293 cell lines expressing hTRPA1, hTRPV1, mTRPV3 and mTRPM8 were seeded in wells of 96-well plates and loaded with Fluo-8. Fluorescence changes were read in a microplate reader while 14a (30 μM) were added at 150 sec before the application of the corresponding agonists: FFA (100 μM) for TRPA1, capsaicin (0.15 μM) for TRPV1, 2-APB (200 μM) for TRPV3, and menthol (100 μM) for TRPM8. Data are means ± SEM for n = 5 measurements normalized to control (in the absence of 14a).