Figure 5.

Overexpression of NSG2 increases mEPSC amplitude. A, Representative traces from whole-cell patch clamp recordings from neurons expressing either mCherry alone (upper trace, black) or NSG2-mC (bottom trace, red). Averaged mEPSCs from both control (black, n = 11) and NSG2-mC (red, n = 10) are shown to the right. B, Pooled data illustrate that neurons expressing NSG2-mC showed a significant increase in mEPSC amplitude compared to controls (**p = 0.01), whereas the frequency of mEPSCs was not different between groups (p = 0.35). C, Neither GluA1⁺ punctae number (p = 0.58) nor relative fluorescence intensity (p = 0.36) were significantly different between groups (mCherry vs NSG2-mC). D, Representative confocal images showing neurons expressing either mCherry alone (left panels) or NSG2-mC (right panels) stained for MAP2 (magenta) and surface GluA2 (cyan). Individual GluA2 and MAP2 expression from both groups (top two panels) are presented in grayscale for clarity. E, Pooled data revealed the number of GluA2⁺ punctae was significantly increased in neurons expressing NSG2-mC (n = 10) compared to controls (n = 10; *p = 0.02), while relative fluorescence tntensity remained unchanged (p = 0.19). Bars represent mean ± SEM; A.U., arbitrary units. Scale bars = 2 µm.