Table 2. The 42 ISSR primers used, along with their respective sequences and parameters in Malus domestica Borkh.
Annealing temperature in °C (AT°C), amplification product (AP), total number of amplified fragments (TN), percentage of polymorphism (%P), polymorphism information content (PIC), and resolving power (RP). The shaded primers were the nine most informative ones in Malus.
| Primer | Sequencea | AT°C | AP | TN | %P | PIC | RP |
|---|---|---|---|---|---|---|---|
| UBC 807 | (AG)8T | 52 | ✓ | 20 | 45.00 | 0.34 | 3.00 |
| UBC 808 | (AG)8C | 50 | – | – | – | – | – |
| UBC 809 | (AT)8T | 55 | – | – | – | – | – |
| UBC 810 | (GA)8T | 52 | – | – | – | – | – |
| UBC 811 | (GA)8C | 53 | – | – | – | – | – |
| UBC 813 | (CT) 8T | 50 | ✓ | 9 | 44.44 | 0.36 | 2.00 |
| UBC 814 | (CT)8A | 50 | ✓ | 9 | 22.22 | 0.33 | 1.00 |
| UBC 815 | (CT)8G | 53 | ✓ | 11 | 81.81 | 0.42 | 6.20 |
| UBC 817 | (CA)8A | 52 | ✓ | 10 | 70.00 | 0.39 | 4.00 |
| UBC 820 | (GT)8T | 52 | ✓ | 7 | 57.14 | 0.44 | 2.60 |
| UBC 822 | (TC)8A | 55 | ✓ | 12 | 25.00 | 0.29 | 1.40 |
| UBC 823 | (TC)8C | 55 | ✓ | 13 | 30.76 | 0.43 | 2.80 |
| UBC 824 | (TC)8G | 50 | – | – | – | – | – |
| UBC 826 | (AC)8C | 52 | ✓ | 11 | 54.54 | 0.40 | 4.00 |
| UBC 827 | (AC)8G | 53 | ✓ | 11 | 9.09 | 0.30 | 0.80 |
| UBC 828 | (TG)8A | 50 | – | – | – | – | – |
| UBC 834 | (AG)8YT | 52 | ✓ | 18 | 38.88 | 0.45 | 5.40 |
| UBC 835 | (AG)8YC | 54 | ✓ | 12 | 33.33 | 0.42 | 2.40 |
| UBC 836 | (AG)8YA | 53 | ✓ | 13 | 30.76 | 0.47 | 3.20 |
| UBC 840 | (GA)8YT | 53 | – | – | – | – | – |
| UBC 843 | (CT)8RA | 54 | ✓ | 12 | 66.66 | 0.37 | 4.20 |
| UBC 848 | (CA)8RG | 55 | – | – | – | – | – |
| UBC 852 | (CT)8RA | 52 | ✓ | 6 | 50.00 | 0.41 | 2.00 |
| UBC 855 | (AC)8YT | 55 | ✓ | 14 | 21.42 | 0.47 | 2.40 |
| UBC 856 | (AC)8YA | 55 | ✓ | 4 | 50.00 | 0.46 | 1.60 |
| UBC 857 | (AC)8YG | 54 | ✓ | 12 | 33.33 | 0.44 | 2.00 |
| UBC 858 | (TG)8RT | 52 | ✓ | 8 | 50.00 | 0.37 | 2.00 |
| UBC 859 | (TG)8RC | 55 | – | – | – | – | – |
| UBC 860 | (TG)8RA | 52 | ✓ | 6 | 83.33 | 0.37 | 2.40 |
| UBC 861 | (ACC)6 | 52 | – | – | – | – | – |
| UBC 864 | (ATG)6 | 50 | – | – | – | – | – |
| UBC 866 | C(TCC)5TC | 55 | – | – | – | – | – |
| UBC 868 | (GGA)6 | 50 | ✓ | 22 | 13.63 | 0.39 | 2.00 |
| UBC 873 | (GACA)4 | 50 | ✓ | 16 | 31.25 | 0.40 | 3.00 |
| UBC 878 | GGA(TGGA)3T | 54 | – | – | – | – | – |
| UBC 881 | (GGGGT)3 | 53 | ✓ | 9 | 33.33 | 0.37 | 1.80 |
| UBC 886 | VDV(CT)7 | 55 | – | – | – | – | – |
| UBC 889 | DBD(AC)7 | 52 | ✓ | 6 | 83.33 | 0.18 | 0.40 |
| UBC 890 | VHV(GT)7 | 54 | ✓ | 11 | 9.09 | 0.32 | 0.40 |
| UBC 891 | HVH(TG)7 | 54 | ✓ | 10 | 30.00 | 0.37 | 1.60 |
| UBC 899 | CATGGTGTTGGTCATTGTTCCA | 55 | – | – | – | – | – |
| UBC 900 | ACTTCCCCACAGGTTAACACA | 55 | – | – | – | – | – |
Notes.
a
Y = (C,T); R = (A, G); H = (A, C, T); B = (C, G, T); V = (A, C, G); D = (A, G, T).