Table 1. List of selected antibodies, lectins and pentraxins for N-glycan epitope screening (Note 4).
| Antibody (1st) | Dilution | Epitope(13,14) | Supplier |
|---|---|---|---|
| Anti-HRP from rabbit, 10 mg/ml | 1:10000 | Core α1,3-Fuc/ core β1,2-Xyl |
Sigma |
| Anti-PC (TEPC-15 mouse IgA), 10 mg/ml | 1:200 | PC-Hex(NAc) | Sigma |
| Antibody (2nd) | |||
| Anti-rabbit IgG from goat conjugated with alkaline phosphatase | 1:2000 | Vector Labs | |
| Anti-mouse IgA from goat conjugated with alkaline phosphatase | 1:10000 | Sigma | |
| Pentraxin | |||
| C reactive Protein (CRP) from human plasma (CaCl22.5 mM added) | 1:200 | PC-Hex(NAc) | MP Biochemicals |
| Amyloid P component from human serum (SAP) | 1:200 | PE-Hex(NAc) | Sigma |
| Pentraxin recognition | |||
| Anti-human C reactive protein from rabbit | 1:1000 | Dako | |
| Anti-Amyloid P human serum component IgG from rabbit (anti SAP) |
1:1000 | Calbiochem | |
| Lectin | |||
| Biotinylated Aleuria aurantia lectin | 1:1000 | Core α1,6-Fuc/Lex | Vector Labs |
| Biotinylated wheat germ agglutinin | 1:1000 | β1,4HexNAc/α2,3Sia | Vector Labs |
| Biotinylated peanut agglutinin | 1:1000 | Galβ1,3GalNAc | Vector Labs |
| Lectin recognition | |||
| Anti-biotin from goat conjugated with alkaline phosphatase | 1:10000 | Sigma |