Table 1. Sequences of primer pairs used for amplifying the genes of interest and the internal reference gene (GAPDH).
| Gene_ | Primers (5’-3’) |
|---|---|
| GAPDH | F- ‘AGCCACATCGCTCAGACAC’ R- ‘GCCCAATACGACCAAATCC’ |
| FXR | F- ‘GATGCCTGTAACAAAGAAGCCCC’ R- ‘CACACAGTTGCCCCCGTTTTTAC’ |
| ASBT | F- ‘TGTGTTGGCTTCCTCTGTCAG’ R- ‘GGCAGCATCCTATAATGAGCAC’ |
| SHP | F- ‘TCAACCCCGATGTGCCAGG’ R- ‘GGTCGGAATGGACTTGAGGG’ |
| Bcl-xL | F- ‘TGCAGGTATTGGTGAGTCGG-3’ R- ‘AAGCGTTCCTGGCCCTTTC’ |
Primers were designed by the PROBEFINDER software (https://www.roche-applied-science.com/sis/rtpcr/upl/index.jsp).