Table 2. Extracellularly Added and Intracellularly Produced Itaconate Distribution between Supernatant and Cells in E. coli Culture Grown in LB Medium.
| itaconate
extracellularly added | |||||
|---|---|---|---|---|---|
| molar
concentration (mM)a |
concentration
in cell culture (mg/L) |
||||
| time (h) | extracellular | intracellular | resulting from supernatant | resulting from cells | Total |
| 0 | 2.5b | ndc | 325.253 | nd | 325.253 |
| 6 | 2.454 ± 0.050 | 1.309 ± 0.132 | 319.242 ± 6.437 | 0.685 ± 0.067 | 319.927 ± 6.437 |
| 12 | 2.462 ± 0.059 | 0.551 ± 0.058 | 320.243 ± 7.715 | 0.411 ± 0.074 | 320.654 ± 7.715 |
| itaconate
intracellularly produced | |||||
|---|---|---|---|---|---|
| molar
concentration (mM) |
normalized
concentration in cell culture (mg/L/OD) |
||||
| time (h) | extracellular | intracellular | resulting from supernatant (% of total) | resulting from cells (% of total) | total |
| 0 | nd | nd | nd | nd | nd |
| 18 | 0.071 ± 0.021 | 0.145 ± 0.008 | 2.149 ± 0.638 (98.67) | 0.029 ± 0.003 (1.33) | 2.178 |
| 36 | 0.242 ± 0.117 | 0.241 ± 0.181 | 7.010 ± 2.506 (99.38) | 0.044 ± 0.028 (0.62) | 7.054 |
a
Arithmetic mean ± standard deviation is derived using data of three biological replicates.
b
Itaconate concentration added to cell culture at 0 h time point.
c
Not detected (nd).