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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: J Immunol. 2018 Nov 19;202(1):194–206. doi: 10.4049/jimmunol.1800777

Figure 3. Rab1a regulates generation of IL-1β and IL-18 via NF-κB-dependent production of pro-IL-1β and pro-IL-18.

Figure 3.

(A) Effects of expression of Rab1a WT or Rab1a N124I on IκBα degradation and NF-κB activity following LPS challenge in BMDMs. BMDMs were transfected with Flag-tagged Rab1a WT, Rab1a N124I cDNA, or control vector. At 48 h posttransfection, the macrophages were stimulated with LPS (20 ng/ ml) for 30 min. (B) Effects of NF-κB inhibitor on the production of pro-IL1β and pro-IL-18. BMDMs were transfected with empty vector or Flag-tagged Rab1a WT cDNA. At 48 h posttransfection, the macrophages were primed with LPS (20 ng/ ml) for 4 h and stimulated with ATP (5 mM) for 30 min in the presence of BMS-345541 (3 μM) or vehicle (PBS). (C) The levels of IL-1β and IL-18 in the culture medium were detected by ELISA. Data are representative of three independent experiments. *p<0.05, vs. vector control (LPS+ATP) group; †p<0.05, vs. LPS+ATP group (Rab1a WT), one-way ANOVA.