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. Author manuscript; available in PMC: 2019 Jan 24.
Published in final edited form as: J Am Soc Mass Spectrom. 2012 May 18;23(8):1390–1399. doi: 10.1007/s13361-012-0404-0

Table 1.

Effect of Mobile Phase Additive on the Numbers of Unique Peptides Identified from Digests of the 5-Protein Mixturea

FA + DMSO + mNBA P value1* P value2**
Trypsin 183±91 231±4 166±6 0.0020 0.0345
Elastase 204±29 242±21 189±43 0.0706 0.3195
Pepsin 372±13 409±7 267±24 0.0108 0.0029
a

Unique peptide counts are the average of three technical replicates. Error values given are one standard deviation.

*

P value from a Student’st-test comparing FA alone with FA+DMSO.

**

P value from a Student’st-test comparing FA alone with FA+m-NBA. The DMSO modified phase identifies significantly more peptides at P valueG0.05 and P<0.01 for peptic and tryptic digests, respectively.