| Antibodies |
|
| Rabbit monoclonal anti-Ki67 (1:200) |
Abcam |
Cat#ab16667; RRID AB_302459 |
| Rabbit monoclonal anti-LEF1 (1:100) |
Cell Signaling |
Cat#2286s; RRID AB_10706166 |
| Mouse monoclonal anti-b-catenin (1:1000) |
Sigma-Aldrich |
Cat# C7207; RRID AB_476865 |
| Rabbit polyclonal anti-LHX2 (1:200) |
Millipore |
Cat# ABE1402; RRID AB_2722523 |
| Rabbit polyclonal anti-Keratin10 (1:1000) |
Covance |
Cat# PRB-159P; RRID AB_291580 |
| Rabbit polyclonal anti-Keratin14 (1:1000) |
BioLegend |
Cat#905301; RRID AB_2565048 |
| Rabbit polyclonal anti-Loricrin (1:1000) |
BioLegend |
Cat# PRB-145P; RRID AB_10064155 |
| Rabbit polyclonal anti-Involucrin (1:1000) |
BioLegend |
Cat# PRB-140C; RRID AB_291569 |
| Rabbit polyclonal anti-SOX9 (1:200) |
EMD-Millipore |
Cat#AB5535, RRID:AB_2239761 |
| Mouse monoclonal anti-hair cortex keratin (AE13) (1:100) |
Abcam |
Cat#ab16113, RRID:AB_302268 |
| Mouse monoclonal anti-Keratin15 (1:2000) |
Abnova Corporation |
Cat#MAB10723, RRID:AB_11189389 |
| Goat anti-Mouse IgG, Alexa Fluor 555 (1:1000) |
ThermoFisher |
Cat# A21425; RRID AB_1500751 |
| Goat anti-Rabbit IgG, Alexa Fluor 555 (1:1000) |
ThermoFisher |
Cat# A21428; RRID AB_141784 |
|
| Critical Commercial Assays |
|
| RNeasy Fibrous Tissue Mini Kit |
QIAGEN |
Cat#74704 |
| SuperScript III First-Strand Synthesis System |
ThermoFisher |
Cat#18080–051 |
| Fast SYBR Green Master Mix |
ThermoFisher |
Cat#4385612 |
|
| Experimental Models: Organisms/Strains |
|
| Mouse: Dkk2tm1Dwu, mixed C57BL/6 / FVB/N / SJL strain background |
The Jackson Laboratory |
RRID:IMSR_JAX:030130 |
| Mouse: Axin2tm1Wbm, mixed C57BL/6 / FVB/N / SJL strain background |
The Jackson Laboratory |
RRID:IMSR_JAX:009120 |
| Mouse: Sostdc1lacZ, mixed C57BL/6 / FVB/N / SJL strain background |
Dr. Gabriela Loots, Lawrence Livermore National Laboratory |
RRID:MGI:4360517 |
| Rabbit: New Zealand white (NZW) |
Covance Research Products |
NZ-CTM |
|
| Oligonucleotides |
|
| Please see Table S1
|
N/A |
N/A |
|
| Recombinant DNA |
|
|
In situ hybridization probe for Dkk2 GenBank: NM_020265.4 nt 1141–1970 |
This paper |
N/A |
|
In situ hybridization probe for Dkk2 GenBank: NM_020265.4, nt 1125–1242 |
This paper |
N/A |
|
In situ hybridization probe for Ctnnb1 (β-catenin) GenBank: NM 007614, nt 150–540 |
Zhang et al., 2009 |
N/A |
