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The formation of the methylglyoxal (MGO) conjugated metabolites of 4'-NH2-EGCG in mice. The LC chromatograms and the ESI-MS2 spectra of MGO-NH2-EGCG (m/z 528 [M-H]−) (A) and N-MGO-NH2-EGCG (m/z 510 [M-H]−) (B) of mouse fecal and urinary samples collected from EGCG (1) treated mice, 4'-NH2-EGCG (2) treated mice, and the sample collected from chemical incubation of 4'-NH2-EGCG (2) with MGO in phosphate buffer (pH 7.4) at 37 °C for 3 h.
