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Published in final edited form as: Free Radic Biol Med. 2018 Dec 19;131:332–344. doi: 10.1016/j.freeradbiomed.2018.12.023

Figure 5. — The formation of the malondialdehyde (MDA) and 4-hydroxy-trans-2-nonenal (4-HNE) conjugated metabolites of EGCG and 4'-NH₂-EGCG in mice. (A) The LC chromatograms and the ESI-MS² spectra of mono MDA-EGCG (m/z 529 [M-H]⁻) of mouse fecal samples collected from EGCG (1) treated mice and the sample collected from chemical incubation of EGCG (1) with MDA in phosphate buffer (pH 7.4) at 37 °C for 6 h. The LC chromatograms and the ESI-MS² spectra of MDA-NH₂-EGCG (m/z 528 [M-H]⁻) (B) and N-MDA-4'-NH₂-EGCG (m/z 510 [M-H]⁻) (C) of mouse fecal and urinary samples collected from EGCG (1) treated mice, 4'-NH₂-EGCG (2) treated mice, and the sample collected from chemical incubation of 4'-NH₂-EGCG (2) with MDA in phosphate buffer (pH 7.4) at 37 °C for 6 h. (D) The LC chromatograms and the ESI-MS² spectra of mono HNE-EGCG (m/z 595 [M-H]⁻) of mouse urinary and fecal samples collected from EGCG (1) treated mice and the sample collected from chemical incubation of EGCG (1) with HNE in phosphate buffer (pH 7.4) at 37 °C for 6 h. (E) The LC chromatograms and the ESI-MS² spectra of N-HNE-4'-NH₂-EGCG (m/z 576 [M-H]⁻) of mouse fecal samples collected from 4'-NH₂-EGCG (2) treated mice and the sample collected from chemical incubation of 4'-NH₂-EGCG (2) with HNE in phosphate buffer (pH 7.4) at 37 °C for 6 h.

Figure 5. — The formation of the malondialdehyde (MDA) and 4-hydroxy-trans-2-nonenal (4-HNE) conjugated metabolites of EGCG and 4'-NH₂-EGCG in mice. (A) The LC chromatograms and the ESI-MS² spectra of mono MDA-EGCG (m/z 529 [M-H]⁻) of mouse fecal samples collected from EGCG (1) treated mice and the sample collected from chemical incubation of EGCG (1) with MDA in phosphate buffer (pH 7.4) at 37 °C for 6 h. The LC chromatograms and the ESI-MS² spectra of MDA-NH₂-EGCG (m/z 528 [M-H]⁻) (B) and N-MDA-4'-NH₂-EGCG (m/z 510 [M-H]⁻) (C) of mouse fecal and urinary samples collected from EGCG (1) treated mice, 4'-NH₂-EGCG (2) treated mice, and the sample collected from chemical incubation of 4'-NH₂-EGCG (2) with MDA in phosphate buffer (pH 7.4) at 37 °C for 6 h. (D) The LC chromatograms and the ESI-MS² spectra of mono HNE-EGCG (m/z 595 [M-H]⁻) of mouse urinary and fecal samples collected from EGCG (1) treated mice and the sample collected from chemical incubation of EGCG (1) with HNE in phosphate buffer (pH 7.4) at 37 °C for 6 h. (E) The LC chromatograms and the ESI-MS² spectra of N-HNE-4'-NH₂-EGCG (m/z 576 [M-H]⁻) of mouse fecal samples collected from 4'-NH₂-EGCG (2) treated mice and the sample collected from chemical incubation of 4'-NH₂-EGCG (2) with HNE in phosphate buffer (pH 7.4) at 37 °C for 6 h.