Table 4.
In vitro cytotoxic activity of ruthenium complexes in cell lines transfected with an empty plasmid (HEK293, NIH3T3) or overexpressing ABC transporters (P-gp, MRP1, MRP2, BCRP) after 48 h incubation at 37 °C. Compounds concentration required to inhibit 50% of the cell’s growth (half maximal inhibitory growth concentration or IG50) was measured by MTT assay.
| Half inhibitory growth concentration, IG50 (µM) | ||||
|---|---|---|---|---|
| NIH3T3╱P-gP | HEK293╱MP1 | HEK293╱MRP2 | HEK293╱BCRP | |
| Rul | 28.1±1.2╱67.8±3.5 | 4.8±0.1╱10±0.4 | 4.5±1.0╱4.9±0.9 | 6.7±0.2╱19.1±0.6 |
| Ru2 | 7.3±0.2╱7.3±0.3 | 1.6±0.9╱1.7±1.2 | 5.7±2.3╱3.0±l.2 | 5.6±2.9╱l.l±0.4 |