(A) Hierarchical clustering based on differential transcriptomes between hpNPCs (hp) and panneuronal NPCs (pan).
(B and C) Immunostaining (B) and quantitation (C) of OTX1/2 expression in hpNPCs and pan-NPCs. Arrowheads represent OTX+ NPCs. *p = 0.022.
(D) Schematic of E12 telencephalon showing the cortical hem (red), DG (yellow), and CA primordia (green and blue).
(E) Wnt signaling pathway is upregulated in hpNPCs (padj ≤ 1.87E-06)
(F) Schematic showing hCA3 differentiation paradigm.
(G) Arrowheads show VGLUT1+ (red) and neuronal marker NeuN+ (green) 4–5 WIV hCA3.
(H) Arrowheads show GRIK4+ MAP2+ neurons in hCA3 (4 WIV).
Results are presented as mean ± SEM. n = 3, *p < 0.05. Scale bar, 50 μm. See also Figure S1.