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. Author manuscript; available in PMC: 2019 Jan 24.
Published in final edited form as: Cell Stem Cell. 2018 May 3;22(5):684–697.e9. doi: 10.1016/j.stem.2018.04.009

Figure 6. hCA3s Connect to hDGs.

Figure 6.

(A) Reconstruction of DG-CA3 neuronal connection (left) in microfluidic devices (right).

(B) hCA3s and hDGs were transfected with LV-Grik4-tdTomato (shown in green) and LVProx1-GFP (shown in red), respectively.

(C) Rabies tracing in microfluidic devices containing hCA3 (left) and hDG (right). Postsynaptic hCA3s (yellow arrows) are labeled with LV-Grik4-G-GFP (green; C, left) and rabies virus (green+red+; C, right); rabies virus traces the presynaptic cells (white arrows) in the DG compartment (red).

(D) Schematic showing postsynaptic (green+red+), presynaptic (red) and CALB+ presynaptic (red+blue+) neurons.

(E) hCA3s are marked CA3-specific LV-Grik4-GGFP (green, yellow arrow) in DG-CA3 co-culture in combination with rabies tracing (red). Immunostaining showing hCA3 (green+red+), presynaptic neurons (red), and CALB1+ (blue, white arrows) presynaptic neurons (red+blue+) as traced by rabies virus (red). Scale bar represents 50 μm in (B) and (C) and 10 μm in (E).

See also Figure S5.