Extended Data Figure 2: Mice with regulatory T cells deficient in RISP do not display thymic dysfunction early in life.

a, b, Thymic weight (a) and total thymocyte number (b) observed from 10-day-old RISP KO (n=5) and WT (n=5) mice. c, Absolute cell numbers of double-negative (CD4⁻ CD8⁻, DN), double-positive (CD4⁺ CD8⁺, DP), CD8 single-positive (CD4⁻ CD8⁺, CD8 SP), and CD4 single-positive (CD4⁺ CD8⁻, CD4 SP) populations from the thymuses of 10-day old RISP KO (n=5) and WT (n=5) mice. d, Foxp3-YFP⁺ and Foxp3-YFP⁺ CD4 SP absolute cell numbers in the thymuses of 10-day old RISP KO (n=5) and WT (n=5) animals..e, Representative contour plot of the T_reg cell compartment in the superficial lymph nodes at 3 weeks of age. Contour plots are representative of at least three independent experiments totaling at least 5 mice. Numbers in dot plot quadrants indicate percentage of cells. Images are representative of at least three mice harvested on independent days. Data represent mean ± SD and were analyzed with (a, b) two-tailed t-test (specific p-values displayed in supplemental source data) or with (c, d) multiple two-tailed t-tests using a two-stage linear step-up procedure of Benjamini, Krieger and Yekutieli, with Q = 1%. Each cell type was analyzed individually, without assuming a consistent SD (specific q-values displayed in supplemental source data). All data points on graphs represent individual animals isolated and analyzed on at least 2 separate days.