REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-YAP	Santa Cruz Biotechnology	Cat# sc-101199, RRID:AB_1131430
Anti-YAP	Proteintech Group	Cat# 13584-1-AP, RRID:AB_2218915
Anti-CYR61	Santa Cruz Biotechnology	Cat# sc-13100, RRID:AB_2088733
Anti-CTGF	Santa Cruz Biotechnology	Cat# sc-14939, RRID:AB_638805
Anti-GAPDH	Santa Cruz Biotechnology	Cat# sc-25778, RRID:AB_10167668
Anti-CAV1	BD Biosciences	RRID:BD610060
Anti-CAV2	BD Biosciences	RRID:BD610684
Anti-HSP90	BD Biosciences	Cat# 610418, RRID:AB_397798
Anti-panTEAD	Cell Signaling Technology	Cat# 13295, RRID:AB_2687902
Anti-YAP p127	Cell Signaling Technology	Cat# 4911S, RRID:AB_2218913
Anti-YAP p397	Cell Signaling Technology	Cat# 13619, RRID:AB_2650554)
Anti-NF2	Cell Signaling Technology	Cat# 6995S, RRID:AB_10828709
Anti-LATS1	Cell Signaling Technology	Cat# 3477, RRID:AB_2133513
Anti-LATS2	Cell Signaling Technology	Cat# 5888, RRID:AB_10835233
Anti-CAVIN1	Cell Signaling Technology	RRID:Cat# 69036
Anti-YAP	Abcam	Cat# ab52771, RRID:AB_2219141
Anti-CAVIN1	Abcam	Cat# ab48824, RRID:AB_882224
Anti-GFP	Abcam	Cat# ab6556, RRID:AB_305564
Anti-CAVIN3	Bethyl	Cat# A302-419A, RRID:AB_1907302
Anti-CAVIN2	Atlas Antibodies	Cat# HPA039325, RRID:AB_10805473
Anti-CAV1	Jørgen Vinten, University of Copenhagen	See [59]
Anti-Myc	Cell Signaling Technology	RRID:Cat# 2276
Anti-mouse IgGs	Fisher scientific	RRID:Cat#12102270
Anti-rabbit IgGs	Fisher scientific	RRID:Cat#1805935
Anti-TEAD1	BD Biosciences	Cat# 610923, RRID:AB_398238
Anti-Goat Immunoglobulins/HRP	DAKO	RRID:P044901
Anti-Rabbit Immunoglobulins/HRP	DAKO	RRID:P044801
Anti-Mouse Immunoglobulins/HRP	DAKO	RRID:P044701
Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488	Thermo Fisher Scientific	Cat# A-11008, RRID:AB_143165
Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 594	Thermo Fisher Scientific	Cat# A-11037, RRID:AB_2534095
Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 594	Thermo Fisher Scientific	Cat# A-11032, RRID:AB_2534091
Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488	Thermo Fisher Scientific	Cat# A-11029, RRID:AB_2534088
Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 633	Thermo Fisher Scientific	Cat# A-21071, RRID:AB_2535732
Bacterial and Virus Strains
Dh5-alpha E.coli	Sonja Vermeren, University of Edinburgh	N/A
One Shot Stbl3 Chemically Competent E. coli	Life Technologies	C737303
Chemicals, Peptides, and Recombinant Proteins
Verteporfin	Sigma-Aldrich	SML0534-5MG
Puromycin	VWR	CAYM13884
G418	Scientific Laboratory Supplies Ltd	G5013
Hygromycin B	Scientific Laboratory Supplies Ltd	30-240-CR
GeneJet DNA transfection reagent	Tebu-Bio	189SL100488
LIPOD293 DNA transfection reagent	Tebu-Bio	189SL100668
DMEM	GIBCO	21969-035
FBS, heat inactivated	GIBCO	10500-064
Glutamine	GIBCO	25030-024
Penicillin/Streptomycin	GIBCO	15140-122
Immuno Western ECL mix	millipore	WBKLS0500
Antifade	citifluor	AF1-100
DMSO	Sigma	D2650
ProLong Diamond Antifade Mountant with DAPI	Thermo Fisher Scientific	P36962
Hoechst	Thermo Fisher Scientific	3342
Formaldehyde Solution, Methanol free	Thermo Fisher Scientific	28908
Polyacrylamide	Thermo Fisher Scientific	BP1408-1
Critical Commercial Assays
RNeasy plus mini kit	QIAGEN	74136
High-capacity cDNA reverse transcriptase kit	Applied biosciences	4368814
Brilliant III Ultra-fast SYBR Green QPCR Master Mix	Agilent Technologies	600883
Dual-Glo luciferase assay system	Promega	E2920
ChIP-IT Express Enzymatic kit	Active Motif Inc.	53009
DNA Clean & Concentrator-5	Zymo Research	D4003T
Experimental Models: Cell Lines
HEK293A	Kun-Liang Guan lab, UCSD	N/A
HEK293T	Kun-Liang Guan lab, UCSD	N/A
U2OS	Jack Dixon, UCSD	N/A
Experimental Models: Organisms/Strains
wwtr1 mutant zebrafish	Link Lab, Medical College of Wisconsin	Allele mw49
yap1 mutant zebrafish	Link Lab, Medical College of Wisconsin	Allele mw48
cav1 mutant zebrafish	Poss Lab, Duke University	Allele pd1104
cav3 mutant zebrafish	Bagnat Lab, Duke University	Allele pd1149
Oligonucleotides
Please refer to Tables S1 and S2	N/A	N/A
Recombinant DNA
Please refer to Table S3	N/A	N/A
Software and Algorithms
Fiji	ImageJ	https://fiji.sc/
Prism	GraphPad	https://www.graphpad.com/scientific-software/prism/
Photoshop and InDesign	Adobe	https://www.adobe.com/uk/creativecloud.html
Excel and Word	Microsoft	https://www.office.com/
BioRender	BioRender	https://app.biorender.io/
Other
Quasi Vivo QV500 system	Kirkstall	QV500
PF22X0103 peristaltic pump	Parker	PARKER PF22X0103
Thermanox 13mm coverslips	Thermo Fisher Scientific	#174950
LightCycler 96 System	Roche	N/A
X Ray Film 18x24cm Double Sided	Scientific Laboratory Supplies Ltd	MOL7016
ThermanoxTM 13 mm coverslips	Thermo Fisher Scientific	#174950
COVER GLASS ROUND Ø 13 MM NO.1	VWR International Ltd.	631-1578
Immobilon(R)-P Polyvinylidene difluoride membranes,size 26.5 cm x 375 cm	Sigma-Aldrich	P2938-1ROL