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. Author manuscript; available in PMC: 2019 Jan 24.
Published in final edited form as: Gynecol Oncol. 2018 Sep 3;151(2):337–344. doi: 10.1016/j.ygyno.2018.08.008

Fig. 3.

Fig. 3.

Mitotic arrest induces apoptosis. A) Western blot analysis of mitotic and apoptotic markers in ARK1 and OVCAR8 cells treated with DMSO or 2 nM SQ1274 for 24 h. B) Representative images and quantitation of apoptotic OVCAR8 cells treated with DMSO, paclitaxel, or SQ1274 detected by staining with DAPI and microscopic analysis of nuclear morphology. Arrow, abnormal DNA condensation; arrowhead, nuclear blebbing. **, P < 0.01; ***, P < 0.001. C) Quantitation of apoptotic ARK1 and OVCAR8 cells treated with DMSO, paclitaxel, or SQ1274 detected by staining with Annexin V/7-AAD and flow cytometric analysis. The mean apoptosis percentage ± SD are plotted, *, P < 0.05; ***, P < 0.001.