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. 2019 Jan 18;9:1991. doi: 10.3389/fpls.2018.01991

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Copyright © 2019 Yuan, Wang, Huang, Xuan and Gao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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PLDδ suppresses RPM1(D505V) induced cell death in N. benthamiana. (A) Trypan blue staining of the infiltrated leaves of N. benthamiana. 35S::PLDδ-YFP-HA or 35S::YFP-HA were co-expressed with Est::RPM1(D505V)-Myc. RPM1(D505V) was induced with 20 μM estradiol at 2 days after infiltration and the leaves were stained at 6 h after induction. Leaves transiently expressed 35S::PLDδ-YFP-HA or 35S::YFP-HA were stained at 2 days after infiltration. (B) Ion-leakage assay. Leaf disks were collected from estradiol induced leaves and floated in distilled water. The conductivity of the water was measured at indicated time points. Each assay was performed with three duplicates. The error bars represent 2X standard deviation (SD). Leaves transiently expressed 35S::YFP-HA were used as control.