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. 2019 Jan 18;9:1991. doi: 10.3389/fpls.2018.01991

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Copyright © 2019 Yuan, Wang, Huang, Xuan and Gao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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PLDδ activity is required to negatively regulate the function and the protein level of RPM1(D505V) in N. benthamiana. (A) PLDδ(R410D/R622P) is deficient to suppress the autoactivity of RPM1(D505V). Est::RPM1(D505V)-Myc was co-expressed with 35S::PLDδ-YFP-HA, 35S::PLDδ(R410D/R622P)-YFP-HA, or 35S::YFP-HA in the leaves of N. benthamiana. RPM1(D505V) was induced with estradiol at 2 days after infiltration. The autoactivity of RPM1(D505V) in the samples was measured with ion-leakage. (B) PLDδ(R410D/R622P) is deficient to reduce the protein level of RPM1(D505V). Samples were collected at 5 h after induction. The protein levels of RPM1(D505V)-Myc, PLDδ(R410D/R622P)-YFP-HA, and PLDδ-YFP-HA were detected. Actin was used as a marker for protein equal loading.