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. 2019 Jan 18;9:1991. doi: 10.3389/fpls.2018.01991

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Copyright © 2019 Yuan, Wang, Huang, Xuan and Gao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Abscisic acid (ABA)-induced PLD activity negatively regulates the protein level of RPM1. (A) ABA treatment reduces the protein level of RPM1. The leaves of pRPM1::RPM1-Myc/rpm1plants were incubated with or without 100 μM of ABA. Protein samples were collected at 3 h after ABA treatment. Three duplicates were detected. (B) n-butanol suppresses ABA-induced RPM1reduction. Plant leaves were incubated with 100 μM of ABA and 1% n-butanol. The leaves were incubated with 1% n-butanol were used as the negative control. The protein levels of RPM1-Myc were compared between the two treatments. Three duplicates were detected. The original western blots are available in Supplementary Materials.