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. 2018 Sep 17;12(1):121–130. doi: 10.1007/s12195-018-0555-6

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Neutrophil transition from rolling to arrest on E-selectin/ICAM-1 and HMVECs. E-selectin/ICAM-1 substrates or inflamed HMVECs were treated with EC-SEAL (30 µM) or GMI-1070 (6.5 µM). Neutrophils were added to the inlet reservoir of a microfluidic device and a syringe pump applied negative pressure at the outlet to create a shear stress of 2 dynes/cm² in the flow chamber. Positional tracking and manual counts allowed for distinguishing between rolling vs. arrested vs. migrated neutrophils. On E-selectin/ICAM-1 substrates, EC-SEAL significantly reduced neutrophil transition to arrest compared to vehicle control, while neither treatment reduced migration of arrested neutrophils. On inflamed HMVECs, both EC-SEAL and GMI-1070 reduced neutrophil transition to arrest and subsequent migration. n = 4, p < 0.05.