Figure 3.

Leish-Box screening results for intracellular L. donovani amastigotes. Leish-Box compounds were tested at 10 µM (A and B, left panels) and 1 µM (A and B, right panels). All 188 compounds were classified according to defined categories. Anti-leishmanial activity is represented as Log10 of mean percentages of amastigotes survival compared to DMSO control as determined in quadruplicate experiments. Inactive, grey; weakly active, purple; active-toxic, orange; active, blue; anti-leishmanial positive control AmB (0.8% survival), red. The outer circle delineates 50% parasite survival and the inner circle delineates the 0.8% survival rate observed for AmB to emphasize very active compounds. Toxic compounds were excluded from this graph (35 and 9 compounds, at 10 and 1 µM, respectively, see Table 1). (C) Stacked bar plot displaying the level of infection observed under different culture conditions denoted on the x-axis. BMDMs were cultured without vehicle (w/o), in presence of DMSO or amphotericin B (AmB). The mean of all 23 active and 7 weakly active compounds at 10 µM is represented.