Figure 4.

Leish-Box screening results for cultured L. donovani promastigotes. A resazurin-based viability assay was used to assess anti-leishmanial activity of Leish-Box compounds against Ld1S promastigotes. Two days after compound addition to parasite cultures at logarithmic growth phase, resazurin was added for an additional 24 hours before reading the level of resofurin fluorescence using an Infinite™ M200 plate reader (Tecan™). Anti-leishmanial activity of compounds are expressed as percent of untreated control (POC) of promastigote survival and displayed in circular plots for compound concentrations of 20 µM (A), 4 µM (B) and 0.8 µM (C). Inactive compounds (POC > 80%), grey; weakly active compounds (50 < POC < 80), purple; active compounds (POC < 50%), blue; AmB, red. The outer and inner circles represent respectively 80% and 50% threshold activity values.