Figure 4.

Effects of RAF inhibitors on the MAPK pathway in cancer cell lines. Phospho-MEK1/2, phospho-ERK1/2, and GAPDH levels in COLO 205 cells (a–d), AsPC-1 cells (e–h), A549 cells (i–l), A427 (m–p), and H-1975 cells (q–t) treated with dabrafenib, LY3009120, TAK-632, or PLX7904. Cells were treated with the indicated concentrations of inhibitors for 2 hours, and cell lysates were analysed by western blotting. Solid black lines represent the boundaries of images cropped from different blots. The membranes were cut out before blotting, and full-length blots are unavailable. Uncropped images are shown in Supplementary Fig. S5.