Fig. 6.

Hippo signalling is disrupted in GSK3 deficient podocytes in vitro. a Summary of proteomics results for Ajuba in Cre treated wild-type (CreWT n = 3), Cre treated floxed GSK3α/β (GSK3KO n = 4) and non-Cre treated floxed GSK3α/β (GSK3FL n = 3) podocytes. ANOVA p = 0.004, Tukey post hoc analysis **p < 0.01 ***p < 0.001. b Representative western blot of analysis of ciGSK3DKO cells shows increased expression of Ajuba relative to controls, n = 3–4. See also Supplementary Fig. 7a. c, d Representative western blots of wild-type mouse podocytes incubated with 20 mM LiCl (c) and 3 μM CHIR99201 (d) showing increased expression of ajuba and Cyclin B1, n = 3. See also Supplementary Figs. 7b and c. e–g Immunofluorescence analysis showing increased nuclear YAP/TAZ staining in ciGSK3DKO cells 24 h after induction of gene knockout (e) n = 3, wild-type podocytes after 24 h 20 mM LiCl (f) n = 4 and 24 h 3 μM CHIR99201 (g) n = 8. Unpaired t test *p < 0.05. Data are presented as the mean ± SEM