Figure 7.

Hypoculoside but not hypoculine disrupts the vacuolar structure of yeast cells. Wild type yeast cells containing vacuoles pre-labelled with the lipophilic dye FM 4-64 were exposed to either DMSO or hypoculoside or hypoculine at the indicated concentrations for 1 hour. Effects of the compounds on vacuolar morphology was analyzed by fluorescence microscopy. (A) Based on the FM 4–64 staining patterns, yeast cells were classified into three categories: Single vacuole or multiple vacuoles or defective vacuoles. Representative images for each category are depicted. (B) Quantification of the various categories for cells treated with either DMSO or hypoculoside or hypoculine is plotted (N = 100 cells). Data from an independent experiment are presented in Supplementary Fig. S13.